Pseudomonas aeruginosa associated pulmonary infections and in vitro amplification virulent rhamnolipid (rhlR) gene

Abstract Background Pseudomonas aeruginosa is a common opportunistic pathogenic bacterium with the ability to develop strong communication pathway by quorum sensing system and different virulent factors. Among various important secretions of P. rhamnolipid biological detergent, believed be involved in development biofilm intercellular communication. It readily dissolves lung surfactants that are then easily catalyzed phospholipases this way acute pulmonary infection. Objective research work was designed investigate virulence gene associated responsible for infections. Methods In current study polymerase chain reaction (PCR) used detection rhlR (rhamnolipid encoding) isolated strains. A number assays were performed ensured its behavior. Disc diffusion method check antibiotic resistance. Isolated strains resistant antibiotics applied. Result found males more prone respiratory infections as compared females. Male members age 44-58 59-73 at higher risk, while females also risk Antibiotic resistance observed measuring zone inhibition GCU-SG-M4, GCU-SG-M3, GCU-SG-M5, GCU-SG-M2, GCU-SG-M1 GCU-SG-M6. GCU-SG-M2 fluconazole (FLU), clarithromycin (CLR), cefixime (CFM) Penicillin (P10). No observed. But it showed unusual diffused around Ak MEM discs. rhl R 16s rRNA characterized analyzed. Conclusion Findings from would help raising awareness about aeruginosa, sequence can diagnostic marker identify samples when sputum Pneumonia patients.